Expanded population of low-density neutrophils in juvenile idiopathic arthritis.

ABSTRACT:

Introduction

Juvenile idiopathic arthritis (JIA), a clinically variable disease characterized by autoimmune arthritis, affects children, and its immunopathology remains elusive. Alterations in neutrophil biology play an important role in this disease. In the present study, we aimed to explore the features of low-density neutrophils (LDNs) in patients with JIA.

Methods

Gene expression of peripheral blood mononuclear cells (PBMCs) from children with distinct subtypes of JIA was analyzed by NanoString Immunology panel. Presence of LDNs was ascertained by flow cytometry and the release of neutrophil-associated products were analyzed by LUMINEX.

Results

LDNs were detected in patients' peripheral blood mononuclear cells (PBMCs) after density gradient centrifugation. Transcr

SUBMITTER: Parackova Z 

PROVIDER: S-EPMC10616245 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature