Investigation of nano- and microdomains formed by ceramide 1 phosphate in lipid bilayers.

ABSTRACT: Biological membranes are renowned for their intricate complexity, with the formation of membrane domains being pivotal to the successful execution of numerous cellular processes. However, due to their nanoscale characteristics, these domains are often understudied, as the experimental techniques required for quantitative investigation present significant challenges. In this study we employ spot-variation z-scan fluorescence correlation spectroscopy (svzFCS) tailored for artificial lipid vesicles of varying composition and combine this approach with high-resolution imaging. This method has been harnessed to examine the lipid-segregation behavior of distinct types of ceramide-1-phosphate (C1P), a crucial class of signaling molecules, within these membranes. Moreover, we provide a quantitativ

SUBMITTER: Drabik D 

PROVIDER: S-EPMC10616280 | biostudies-literature | 2023 Oct

REPOSITORIES: biostudies-literature