Exploring intrinsic variability between cultured nasal and bronchial epithelia in cystic fibrosis.

ABSTRACT: The nasal and bronchial epithelium are unified parts of the respiratory tract that are affected in the monogenic disorder cystic fibrosis (CF). Recent studies have uncovered that nasal and bronchial tissues exhibit intrinsic variability, including differences in mucociliary cell composition and expression of unique transcriptional regulatory proteins which relate to germ layer origin. In the present study, we explored whether intrinsic differences between nasal and bronchial epithelial cells persist in cell cultures and affect epithelial cell functioning in CF. Comparison of air-liquid interface (ALI) differentiated epithelial cells from subjects with CF revealed distinct mucociliary differentiation states of nasal and bronchial cultures. Moreover, using RNA sequencing we identified cell t

SUBMITTER: Rodenburg LW 

PROVIDER: S-EPMC10616285 | biostudies-literature | 2023 Oct

REPOSITORIES: biostudies-literature