Mucosal immune alterations at the early onset of tissue destruction in chronic obstructive pulmonary disease.

ABSTRACT:

Rationale

COPD is characterized by chronic airway inflammation, small airways changes, with disappearance and obstruction, and also distal/alveolar destruction (emphysema). The chronology by which these three features evolve with altered mucosal immunity remains elusive. This study assessed the mucosal immune defense in human control and end-stage COPD lungs, by detailed microCT and RNA transcriptomic analysis of diversely affected zones.

Methods

In 11 control (non-used donors) and 11 COPD (end-stage) explant frozen lungs, 4 cylinders/cores were processed per lung for microCT and tissue transcriptomics. MicroCT was used to quantify tissue percentage and alveolar surface density to classify the COPD cores in mild, moderate and severe alveolar destruction groups, as well as to

SUBMITTER: de Fays C 

PROVIDER: S-EPMC10616299 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature