Exosomes from magnetic particles-primed mesenchymal stem cells enhance neural differentiation of PC12 cells.

ABSTRACT: This study aimed to investigate the effects of mesenchymal stem cell exosomes loaded with Fe₃O₄ magnetic particles (Fe₃O₄@ MSC-exo) on the survival and neural differentiation of PC12 cells. Exosomes were separated from Fe₃O₄ magnetic nanoparticles-primed umbilical cord mesenchymal stem cells condition medium by ultracentrifugation and characterized by transmission electron microscopy, flow nano analysis, and western blotting. PC12 cells were treated with culture medium containing exosomes. The effects of Fe₃O₄@ MSC-exo on PC12 cell proliferation, migration, and neural differentiation were analyzed using CCK-8 assay, transwell migration assay, RT-qPCR, and immunofluorescence, respectively. Additionally,

SUBMITTER: Xie Y 

PROVIDER: S-EPMC10616344 | biostudies-literature | 2023 Oct

REPOSITORIES: biostudies-literature