Identification of developmentally specific enhancers for Tsix in the regulation of X chromosome inactivation.

ABSTRACT: X chromosome inactivation silences one of two X chromosomes in the mammalian female cell and is controlled by a binary switch that involves interactions between Xist and Tsix, a sense-antisense pair of noncoding genes. On the future active X chromosome, Tsix expression suppresses Xist upregulation, while on the future inactive X chromosome, Tsix repression is required for Xist-mediated chromosome silencing. Thus, understanding the binary switch mechanism depends on ascertaining how Tsix expression is regulated. Here we have taken an unbiased approach toward identifying Tsix regulatory elements within the X chromosome inactivation center. First, we defined the major Tsix promoter and found that it cannot fully recapitulate the developmental dynamics of Tsix expression, indicating a requirem

SUBMITTER: Stavropoulos N 

PROVIDER: S-EPMC1061649 | biostudies-literature | 2005 Apr

REPOSITORIES: biostudies-literature