Integration of circulating microRNAs and transcriptome signatures identifies early-pregnancy biomarkers of preeclampsia.

ABSTRACT:

Background

MicroRNAs (miRNAs) have been implicated in the pathobiology of preeclampsia, a common hypertensive disorder of pregnancy. In a nested matched case-control cohort within the Vitamin D Antenatal Asthma Reduction Trial (VDAART), we previously identified peripheral blood mRNA signatures related to preeclampsia and vitamin D status (≤30 ng/mL) during gestation from 10 to 18 weeks, using differential expression analysis.

Methods

Using quantitative PCR arrays, we conducted profiling of circulating miRNAs at 10-18 weeks of gestation in the same VDAART cohort to identify differentially expressed (DE) miRNAs associated with preeclampsia and vitamin D status. For the validation of the expression of circulating miRNA signatures in the placenta, the HTR-8/SVneo trophoblast cel

SUBMITTER: Mirzakhani H 

PROVIDER: S-EPMC10616748 | biostudies-literature | 2023 Nov

REPOSITORIES: biostudies-literature