Single-Particle Plasmon Sensor to Monitor Proteolytic Activity in Real Time.

ABSTRACT: We have established a label-free plasmonic platform that monitors proteolytic activity in real time. The sensor consists of a random array of gold nanorods that are functionalized with a design peptide that is specifically cleaved by thrombin, resulting in a blueshift of the longitudinal plasmon. By monitoring the plasmon of many individual nanorods, we determined thrombin's proteolytic activity in real time and inferred relevant kinetic parameters. Furthermore, a comparison to a kinetic model revealed that the plasmon shift is dictated by a competition between peptide cleavage and thrombin binding, which have opposing effects on the measured plasmon shift. The dynamic range of the sensor is greater than two orders of magnitude, and it is capable of detecting physiologically relevant level

SUBMITTER: Oliveira-Silva R 

PROVIDER: S-EPMC10616847 | biostudies-literature | 2023 Oct

REPOSITORIES: biostudies-literature