Automated EuroFlow approach for standardized in-depth dissection of human circulating B-cells and plasma cells.

ABSTRACT:

Background

Multiparameter flow cytometry (FC) immunophenotyping is a key tool for detailed identification and characterization of human blood leucocytes, including B-lymphocytes and plasma cells (PC). However, currently used conventional data analysis strategies require extensive expertise, are time consuming, and show limited reproducibility.

Objective

Here, we designed, constructed and validated an automated database-guided gating and identification (AGI) approach for fast and standardized in-depth dissection of B-lymphocyte and PC populations in human blood.

Methods

For this purpose, 213 FC standard (FCS) datafiles corresponding to umbilical cord and peripheral blood samples from healthy and patient volunteers, stained with the 14-color 18-antibody EuroFlow BIgH-IM

SUBMITTER: Delgado AH 

PROVIDER: S-EPMC10616957 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature