Preadipocytes in human granulation tissue: role in wound healing and response to macrophage polarization.

ABSTRACT:

Background

Chronic non-healing wounds pose a global health challenge. Under optimized conditions, skin wounds heal by the formation of scar tissue. However, deregulated cell activation leads to persistent inflammation and the formation of granulation tissue, a type of premature scar tissue without epithelialization. Regenerative cells from the wound periphery contribute to the healing process, but little is known about their cellular fate in an inflammatory, macrophage-dominated wound microenvironment.

Methods

We examined CD45^-/CD31^-/CD34⁺ preadipocytes and CD68⁺ macrophages in human granulation tissue from pressure ulcers (n=6) using immunofluorescence, immunohistochemistry, and flow cytometry. In vitro, we studied macrophage-prea

SUBMITTER: Rauchenwald T 

PROVIDER: S-EPMC10617061 | biostudies-literature | 2023 Oct

REPOSITORIES: biostudies-literature