In-organoid single-cell CRISPR screening reveals determinants of hepatocyte differentiation and maturation.

ABSTRACT:

Background

Harnessing hepatocytes for basic research and regenerative medicine demands a complete understanding of the genetic determinants underlying hepatocyte differentiation and maturation. Single-cell CRISPR screens in organoids could link genetic perturbations with parallel transcriptomic readout in single cells, providing a powerful method to delineate roles of cell fate regulators. However, a big challenge for identifying key regulators during data analysis is the low expression levels of transcription factors (TFs), which are difficult to accurately estimate due to noise and dropouts in single-cell sequencing. Also, it is often the changes in TF activities in the transcriptional cascade rather than the expression levels of TFs that are relevant to the cell fate transition.

SUBMITTER: Liang J 

PROVIDER: S-EPMC10617096 | biostudies-literature | 2023 Oct

REPOSITORIES: biostudies-literature