Decreased HAT1 expression in granulosa cells disturbs oocyte meiosis during mouse ovarian aging.

ABSTRACT:

Background

With advanced maternal age, abnormalities during oocyte meiosis increase significantly. Aneuploidy is an important reason for the reduction in the quality of aged oocytes. However, the molecular mechanism of aneuploidy in aged oocytes is far from understood. Histone acetyltransferase 1 (HAT1) has been reported to be essential for mammalian development and genome stability, and involved in multiple organ aging. Whether HAT1 is involved in ovarian aging and the detailed mechanisms remain to be elucidated.

Methods

The level of HAT1 in aged mice ovaries was detected by immunohistochemical and immunoblotting. To explore the function of HAT1 in the process of mouse oocyte maturation, we used Anacardic Acid (AA) and small interfering RNAs (siRNA) to culture cumulus-oocyt

SUBMITTER: Guo B 

PROVIDER: S-EPMC10617186 | biostudies-literature | 2023 Oct

REPOSITORIES: biostudies-literature