Identification of cell-type specific alternative transcripts in the multicellular alga Volvox carteri.

ABSTRACT:

Background

Cell type specialization is a hallmark of complex multicellular organisms and is usually established through implementation of cell-type-specific gene expression programs. The multicellular green alga Volvox carteri has just two cell types, germ and soma, that have previously been shown to have very different transcriptome compositions which match their specialized roles. Here we interrogated another potential mechanism for differentiation in V. carteri, cell type specific alternative transcript isoforms (CTSAI).

Methods

We used pre-existing predictions of alternative transcripts and de novo transcript assembly with HISAT2 and Ballgown software to compile a list of loci with two or more transcript isoforms, identified a small subset that were candidates for CTSAI,

SUBMITTER: Balasubramanian RN 

PROVIDER: S-EPMC10617192 | biostudies-literature | 2023 Oct

REPOSITORIES: biostudies-literature