Proviral location affects cognate peptide-induced virus production and immune recognition of HIV-1-infected T cell clones.

ABSTRACT: BACKGROUNDHIV-1-infected CD4+ T cells contribute to latent reservoir persistence by proliferating while avoiding immune recognition. Integration features of intact proviruses in elite controllers (ECs) and people on long-term therapy suggest that proviruses in specific chromosomal locations can evade immune surveillance. However, direct evidence of this mechanism is missing.METHODSIn this case report, we characterized integration sites and full genome sequences of expanded T cell clones in an EC before and after chemoradiation. We identified the cognate peptide of infected clones to investigate cell proliferation and virus production induced by T cell activation, and susceptibility to autologous CD8+ T cells.RESULTSThe proviral landscape was dominated by 2 large clones with replication-com

SUBMITTER: Dragoni F 

PROVIDER: S-EPMC10617777 | biostudies-literature | 2023 Nov

REPOSITORIES: biostudies-literature