SHED-derived exosomes ameliorate hyposalivation caused by Sjogren's syndrome via Akt/GSK-3β/Slug-mediated ZO-1 expression.

ABSTRACT:

Background

Sjögren's syndrome (SS) is an autoimmune disorder characterized by sicca syndrome and/or systemic manifestations. The treatment is still challenging. This study aimed to explore the therapeutic role and mechanism of exosomes obtained from the supernatant of stem cells derived from human exfoliated deciduous teeth (SHED-exos) in sialadenitis caused by SS.

Methods

SHED-exos were administered to the submandibular glands (SMGs) of 14-week-old non-obese diabetic (NOD) mice, an animal model of the clinical phase of SS, by local injection or intraductal infusion. The saliva flow rate was measured after pilocarpine intraperitoneal injection in 21-week-old NOD mice. Protein expression was examined by western blot analysis. Exosomal microRNA (miRNAs) were identified by micr

SUBMITTER: Du Z 

PROVIDER: S-EPMC10617935 | biostudies-literature | 2023 Nov

REPOSITORIES: biostudies-literature