Energy coupling and stoichiometry of Zn²⁺/H⁺ antiport by the prokaryotic cation diffusion facilitator YiiP.

ABSTRACT: YiiP from Shewanella oneidensis is a prokaryotic Zn²⁺/H⁺ antiporter that serves as a model for the Cation Diffusion Facilitator (CDF) superfamily, members of which are generally responsible for homeostasis of transition metal ions. Previous studies of YiiP as well as related CDF transporters have established a homodimeric architecture and the presence of three distinct Zn²⁺ binding sites named A, B, and C. In this study, we use cryo-EM, microscale thermophoresis and molecular dynamics simulations to address the structural and functional roles of individual sites as well as the interplay between Zn²⁺ binding and protonation. Structural studies indicate that site C in the cytoplasmic domain is primarily responsible for stabilizing the dimer and tha

SUBMITTER: Hussein A 

PROVIDER: S-EPMC10617992 | biostudies-literature | 2023 Oct

REPOSITORIES: biostudies-literature