Down-regulation of DPP4 by TGFβ1/miR29a-3p inhibited proliferation and promoted migration of ovarian cancer cells.

ABSTRACT:

Objective

To explore the DPP4 expression changes and functions in ovarian cancer (OV), as well as the regulation mechanism for DDP4.

Methods

GEPIA2, GSE18520, GSE26712 and UALCAN were used to analyze differences in DPP4 expression between OV tumors and control tissues. Serum DPP4 levels were measured by ELISA. The prognostic values of DPP4 were evaluated using a Kaplan-Meier (KM) plotter. Small interfering RNA was used for DPP4 knockdown in OVCAR-3 and SKOV-3 cells. CCK-8 and scratch healing assays were used to determine the cells' proliferation and migration abilities. Flow cytometry (FCM) was used to detect the cell cycle and apoptosis. A dual-luciferase assay was designed to confirm the regulatory effect of miR-29a-3p on DPP4.

Results

The expressions of DPP4 mRNA a

SUBMITTER: Liu C 

PROVIDER: S-EPMC10618141 | biostudies-literature | 2023 Oct

REPOSITORIES: biostudies-literature