Molecular imaging of bacterial outer membrane vesicles based on bacterial surface display.

ABSTRACT: The important roles of bacterial outer membrane vesicles (OMVs) in various diseases and their emergence as a promising platform for vaccine development and targeted drug delivery necessitates the development of imaging techniques suitable for quantifying their biodistribution with high precision. To address this requirement, we aimed to develop an OMV specific radiolabeling technique for positron emission tomography (PET). A novel bacterial strain (E. coli BL21(DE3) ΔnlpI, ΔlpxM) was created for efficient OMV production, and OMVs were characterized using various methods. SpyCatcher was anchored to the OMV outer membrane using autotransporter-based surface display systems. Synthetic SpyTag-NODAGA conjugates were tested for OMV surface binding and ⁶⁴Cu labeling efficiency. The fin

SUBMITTER: Szollosi D 

PROVIDER: S-EPMC10618197 | biostudies-literature | 2023 Oct

REPOSITORIES: biostudies-literature