Development and characterization of a bacteriophage cocktail with high lytic efficacy against field-isolated Salmonella enterica.

ABSTRACT: Salmonella spp. is a prevalent pathogen that causes great public health concern worldwide. Bacteriophage-based cocktails have arisen as an alternative to antibiotics to inhibit the growth of Salmonella. However, the bactericidal effect of bacteriophage cocktails in vivo largely differs from their observed effect in vitro. This is partly because in vitro developments of cocktails do not always consider the bacterial diversity nor the environmental conditions where bacteriophages will have to replicate. Here, we isolated and sequenced 47 bacteriophages that showed variable degrees of lytic activity against 258 Salmonella isolates from a commercial broiler company in Brazil. Three of these bacteriophages were characterized and selected to assemble a cocktail. In vitro quantitative assays dete

SUBMITTER: Aguilera M 

PROVIDER: S-EPMC10618821 | biostudies-literature | 2023 Sep

REPOSITORIES: biostudies-literature