Insight into the structures of unusual base pairs in RNA complexes containing a primer/template/adenosine ligand.

ABSTRACT: In the prebiotic RNA world, the self-replication of RNA without enzymes can be achieved through the utilization of 2-aminoimidazole activated nucleotides as efficient substrates. The mechanism of RNA nonenzymatic polymerization has been extensively investigated biophysically and structurally by using the model of an RNA primer/template complex which is bound by the imidazolium-bridged or triphosphate-bridged diguanosine intermediate. However, beyond the realm of the guanosine substrate, the structural insight into how alternative activated nucleotides bind and interact with the RNA primer/template complex remains unexplored, which is important for understanding the low reactivity of adenosine and uridine substrates in RNA primer extension, as well as its relationship with the structures. H

SUBMITTER: Dantsu Y 

PROVIDER: S-EPMC10619131 | biostudies-literature | 2023 Nov

REPOSITORIES: biostudies-literature