Imaging and detecting intercellular tensile forces in spheroids and embryoid bodies using lipid-modified DNA probes.

ABSTRACT: Cells continuously experience and respond to different physical forces that are used to regulate their physiology and functions. Our ability to measure these mechanical cues is essential for understanding the bases of various mechanosensing and mechanotransduction processes. While multiple strategies have been developed to study mechanical forces within two-dimensional (2D) cell culture monolayers, the force measurement at cell-cell junctions in real three-dimensional (3D) cell models is still pretty rare. Considering that in real biological systems, cells are exposed to forces from 3D directions, measuring these molecular forces in their native environment is thus highly critical for the better understanding of different development and disease processes. We have recently developed a type

SUBMITTER: Tian Q 

PROVIDER: S-EPMC10619156 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature