Extracellular matrix modulates the spatial hepatic features in hepatocyte-like cells derived from human embryonic stem cells.

ABSTRACT:

Background

Human pluripotent stem cell (hPSC)-derived hepatocyte-like cells (HLCs) can provide a valuable in vitro model for disease modelling and drug development. However, generating HLCs with characteristics comparable to hepatocytes in vivo is challenging. Extracellular matrix (ECM) plays an important role in supporting liver development and hepatocyte functions, but their impact on hepatocyte differentiation and maturation during hPSC differentiation remains unclear. Here, we investigate the effects of two ECM components-Matrigel and type I collagen on hepatic differentiation of human embryonic stem cells (hESCs).

Methods

hESC-derived HLCs were generated through multistage differentiation in two-dimensional (2D) and three-dimensional (3D) cultures, incorporating either

SUBMITTER: Farhan F 

PROVIDER: S-EPMC10619266 | biostudies-literature | 2023 Nov

REPOSITORIES: biostudies-literature