The association of Treg and Th17 cells development factors and anti-TPO autoantibodies in patients with recurrent pregnancy loss.

ABSTRACT:

Objectives

Thyroid autoimmunity is considered as the most prevalent autoimmune condition in women in fertility age. There are different clinical evidences indicating the association between thyroid autoimmunity and increased risk of RPL. This study aimed to analyze the association of Tregs and Th17 cells development factors and anti-thyroid peroxidase (anti-TPO) antibodies in RPL patients. Healthy controls (n = 36), TPO + controls (n = 25) and TPO + RPL (n = 32) participated in this study. After blood sampling, the frequency of Th17 and Tregs was evaluated using flow cytometry. Real-time PCR and ELISA was used to assess the status of Tregs and Th17 related transcription factors and cytokines in mRNA and protein level, respectively.

Results

TPO + RPL group showed a higher Th1

SUBMITTER: Niafar M 

PROVIDER: S-EPMC10619307 | biostudies-literature | 2023 Oct

REPOSITORIES: biostudies-literature