Anaerobic fixed-target serial crystallography using sandwiched silicon nitride membranes.

ABSTRACT: In recent years, the emergence of serial crystallography, initially pioneered at X-ray free-electron lasers (XFELs), has sparked a growing interest in collecting macromolecular crystallographic data at room temperature. Various fixed-target serial crystallography techniques have been developed, ranging from commercially available chips to in-house designs implemented at different synchrotron facilities. Nevertheless, there is currently no commercially available chip (known to the authors) specifically designed for the direct handling of oxygen-sensitive samples. This study presents a methodology employing silicon nitride chips arranged in a `sandwich' configuration, enabling reliable room-temperature data collection from oxygen-sensitive samples. The method involves the utilization of a cu

SUBMITTER: Bjelcic M 

PROVIDER: S-EPMC10619425 | biostudies-literature | 2023 Nov

REPOSITORIES: biostudies-literature