Metal organic layers enabled cell surface engineering coupling biomembrane fusion for dynamic membrane proteome profiling.

ABSTRACT: Systematically dissecting the highly dynamic and tightly communicating membrane proteome of living cells is essential for the system-level understanding of fundamental cellular processes and intricate relationship between membrane-bound organelles constructed through membrane traffic. While extensive efforts have been made to enrich membrane proteins, their comprehensive analysis with high selectivity and deep coverage remains a challenge, especially at the living cell state. To address this problem, we developed the cell surface engineering coupling biomembrane fusion method to map the whole membrane proteome from the plasma membrane to various organelle membranes taking advantage of the exquisite interaction between two-dimensional metal-organic layers and phospholipid bilayers on the me

SUBMITTER: Jiang Q 

PROVIDER: S-EPMC10619618 | biostudies-literature | 2023 Nov

REPOSITORIES: biostudies-literature