Hepatitis B surface antigen reduction is associated with hepatitis B core-specific CD8⁺ T cell quality.

ABSTRACT: Despite treatment, hepatitis B surface antigen (HBsAg) persists in patients with chronic hepatitis B (CHB), suggesting the likely presence of the virus in the body. CD8⁺ T cell responses are essential for managing viral replication, but their effect on HBsAg levels remains unclear. We studied the traits of activated CD8⁺ T cells and HBV-specific CD8⁺ T cells in the blood of CHB patients undergoing nucleos(t)ide analog (NUC) therapy. For the transcriptome profiling of activated CD8⁺ T cells in peripheral blood mononuclear cells (PBMCs), CD69⁺ CD8⁺ T cells were sorted from six donors, and single-cell RNA sequencing (scRNA-seq) analysis was performed. To detect HBV-specific CD8⁺ T cells, we stimulated PBMCs from 26 d

SUBMITTER: Takahama S 

PROVIDER: S-EPMC10619684 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature