Microbial thermogenesis is dependent on ATP concentrations and the protein kinases ArcB, GlnL, and YccC.

ABSTRACT: Organisms necessarily release heat energy in their pursuit of survival. This process is known as cellular thermogenesis and is implicated in many processes from cancer metabolism to spontaneous farm fires. However, the molecular basis for this fundamental phenomenon is yet to be elucidated. Here, we show that the major players involved in the cellular thermogenesis of Escherichia coli are the protein kinases ArcB, GlnL, and YccC. We also reveal the substrate-level control of adenosine triphosphate (ATP)-driven autophosphorylation that governs cellular thermogenesis. Specifically, through live cell microcalorimetry, we find these regulatory proteins, when knocked out in a model E. coli strain, dysregulate cellular thermogenesis. This dysregulation can be seen in an average 25% or greater in

SUBMITTER: Dhatt PS 

PROVIDER: S-EPMC10619766 | biostudies-literature | 2023 Oct

REPOSITORIES: biostudies-literature