Cross-kingdom expression of synthetic genetic elements promotes discovery of metabolites in the human microbiome.

ABSTRACT: Small molecules encoded by biosynthetic pathways mediate cross-species interactions and harbor untapped potential, which has provided valuable compounds for medicine and biotechnology. Since studying biosynthetic gene clusters in their native context is often difficult, alternative efforts rely on heterologous expression, which is limited by host-specific metabolic capacity and regulation. Here, we describe a computational-experimental technology to redesign genes and their regulatory regions with hybrid elements for cross-species expression in Gram-negative and -positive bacteria and eukaryotes, decoupling biosynthetic capacity from host-range constraints to activate silenced pathways. These synthetic genetic elements enabled the discovery of a class of microbiome-derived nucleotide metab

SUBMITTER: Patel JR 

PROVIDER: S-EPMC10619838 | biostudies-literature | 2022 Apr

REPOSITORIES: biostudies-literature