Chromatin compartmentalization regulates the response to DNA damage.

ABSTRACT: The DNA damage response is essential to safeguard genome integrity. Although the contribution of chromatin in DNA repair has been investigated^1,2, the contribution of chromosome folding to these processes remains unclear³. Here we report that, after the production of double-stranded breaks (DSBs) in mammalian cells, ATM drives the formation of a new chromatin compartment (D compartment) through the clustering of damaged topologically associating domains, decorated with γH2AX and 53BP1. This compartment forms by a mechanism that is consistent with polymer-polymer phase separation rather than liquid-liquid phase separation. The D compartment arises mostly in G1 phase, is independent of cohesin and is enhanced after pharmacological inhibition of DNA-dependent protein kin

SUBMITTER: Arnould C 

PROVIDER: S-EPMC10620078 | biostudies-literature | 2023 Nov

REPOSITORIES: biostudies-literature