Proteome census upon nutrient stress reveals Golgiphagy membrane receptors.

ABSTRACT: During nutrient stress, macroautophagy degrades cellular macromolecules, thereby providing biosynthetic building blocks while simultaneously remodelling the proteome^1,2. Although the machinery responsible for initiation of macroautophagy has been well characterized^3,4, our understanding of the extent to which individual proteins, protein complexes and organelles are selected for autophagic degradation, and the underlying targeting mechanisms, is limited. Here we use orthogonal proteomic strategies to provide a spatial proteome census of autophagic cargo during nutrient stress in mammalian cells. We find that macroautophagy has selectivity for recycling membrane-bound organelles (principally Golgi and endoplasmic reticulum). Through autophagic cargo prioritization, we

SUBMITTER: Hickey KL 

PROVIDER: S-EPMC10620096 | biostudies-literature | 2023 Nov

REPOSITORIES: biostudies-literature