Diffuse Reflectance-Based Femtosecond Stimulated Raman Spectroscopy of Opaque Suspensions.

ABSTRACT: By augmentation of the collection optics utilized in transmission-based femtosecond stimulated Raman spectroscopy (FSRS), two novel diffuse reflectance-based femtosecond stimulated Raman spectroscopy (drFSRS) techniques were developed. These techniques were then used to collect the Raman spectra of opaque systems, those being cyclohexane-intercalated poly(tetrafluoroethylene) microbeads and ethanol in 1% intralipid solutions. The resulting drFSRS data from the cyclohexane:PTFE system show significant distortion of the depolarization ratio of the 803 cm^-1 cyclohexane peak, indicating a loss of incident pump:probe polarization in a scattering environment. The drFSRS data from the ethanol in 1% intralipid solution demonstrate less signal strength but equal spectral resolution when

SUBMITTER: Diaz SA 

PROVIDER: S-EPMC10620773 | biostudies-literature | 2023 Oct

REPOSITORIES: biostudies-literature