Development of a triplex real-time quantitative PCR for detection and differentiation of genotypes I and II African swine fever virus.

ABSTRACT: African swine fever virus (ASFV) was first identified in 1921 and is extensively prevalent around the world nowadays, which has a significant negative impact on the swine industry. In China, genotype II ASFV was first discovered in 2018, and has spread quickly to different provinces in a very short time; genotype I ASFV was first found in 2020, and has been reported in several provinces since then. To establish an accurate method for detection and differentiation of genotypes I and II ASFV, three primers and probes were designed targeting the ASFV B646L gene for different genotypes, the F1055L gene for genotype I, and the E183L gene for genotype II, and a triplex real-time quantitative PCR (qPCR) for differential detection of genotypes I and II ASFV was developed after optimizing the react

SUBMITTER: Qian X 

PROVIDER: S-EPMC10620837 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature