A facile method to generate cerebral organoids from human pluripotent stem cells.

ABSTRACT: Human cerebral organoids (COs) are self-organizing three-dimensional (3D) neural structures that provide a human-specific platform to study the cellular and molecular processes that underlie different neurological events. The first step of CO generation from human pluripotent stem cells (hPSCs) is neural induction, which is an in vitro simulation of neural ectoderm development. Several signaling pathways cooperate during neural ectoderm development and in vitro differentiation of hPSCs toward neural cell lineages is also affected by them. In this study, we considered some of the known sources of these variable signaling cues arising from cell culture media components and sought to modulate their effects by applying a comprehensive combination of small molecules and growth fac

SUBMITTER: Simorgh S 

PROVIDER: S-EPMC10620858 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature