Dataset Information

Soft Deposition of Organic Molecules Based on Cluster-Induced Desorption for the Investigation of On-Surface and Surface-Mediated Reactions.

ABSTRACT: Desorption/ionization induced by neutral clusters (DINeC) was employed for the soft transfer of organic and biomolecules, such as porphyrins and peptides, from a bulk sample onto any substrate of choice. Qualitative analysis of the deposition technique was performed by means of mass spectrometry, demonstrating that the deposited molecules remained intact due to the soft nature of the transfer process. Deposition rates were studied quantitatively using a quartz crystal microbalance; layers of intact biomolecules ranging from the submonolayer regime up to a few monolayers in thickness were realized. Mixed layers of molecules were deposited when two different sources of molecules were employed. The samples which were prepared based on this soft deposition method were used for the investigation of reactions of the deposited molecules with either coadsorbates on the surface or the surface itself. Examples include adduct formation of peptides with alkali metals on SiO₂, the oxidation of peptides exposed to oxygen, as well as the metallization of porphyrins in interaction with the substrate.

SUBMITTER: Pluschke K

PROVIDER: S-EPMC10620888 | biostudies-literature | 2023 Oct

REPOSITORIES: biostudies-literature

ACCESS DATA

Publications

Soft Deposition of Organic Molecules Based on Cluster-Induced Desorption for the Investigation of On-Surface and Surface-Mediated Reactions.

Pluschke Karolin K Herrmann Aaron A Dürr Michael M

ACS omega 20231019 43

Desorption/ionization induced by neutral clusters (DINeC) was employed for the soft transfer of organic and biomolecules, such as porphyrins and peptides, from a bulk sample onto any substrate of choice. Qualitative analysis of the deposition technique was performed by means of mass spectrometry, demonstrating that the deposited molecules remained intact due to the soft nature of the transfer process. Deposition rates were studied quantitatively using a quartz crystal microbalance; layers of int ...[more]

PMID: 37929133

Similar Datasets

Project description:Recently we have shown that, as a versatile ionization technique, desorption electrospray ionization (DESI) can serve as a useful interface to combine electrochemistry (EC) with mass spectrometry (MS). In this study, the EC/DESI-MS method has been further applied to investigate some aqueous phase redox reactions of biological significance, including the reduction of peptide disulfide bonds and nitroaromatics as well as the oxidation of phenothiazines. It was found that knotted/enclosed disulfide bonds in the peptides apamin and endothelin could be electrochemically cleaved. Subsequent tandem MS analysis of the resulting reduced peptide ions using collision-induced dissociation (CID) and electron-capture dissociation (ECD) gave rise to extensive fragment ions, providing a fast protocol for sequencing peptides with complicated disulfide bond linkages. Flunitrazepam and clonazepam, a class of nitroaromatic drugs, are known to undergo reduction into amines which was proposed to involve nitroso and N-hydroxyl intermediates. Now in this study, these corresponding intermediate ions were successfully intercepted and their structures were confirmed by CID. This provides mass spectrometric evidence for the mechanism of the nitro to amine conversion process during nitroreduction, an important redox reaction involved in carcinogenesis. In addition, the well-known oxidation reaction of chlorpromazine was also examined. The putative transient one-electron transfer product, the chlorpromazine radical cation (m/z 318), was captured by MS, for the first time, and its structure was also verified by CID. In addition to these observations, some features of the DESI-interfaced electrochemical mass spectrometry were discussed, such as simple instrumentation and the lack of background signal. These results further demonstrate the feasibility of EC/DESI-MS for the study of the biology-relevant redox chemistry and would find applications in proteomics and drug development research.

Dataset Information

Soft Deposition of Organic Molecules Based on Cluster-Induced Desorption for the Investigation of On-Surface and Surface-Mediated Reactions.

Publications

Soft Deposition of Organic Molecules Based on Cluster-Induced Desorption for the Investigation of On-Surface and Surface-Mediated Reactions.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets