¹³C tracer analysis reveals the landscape of metabolic checkpoints in human CD8⁺ T cell differentiation and exhaustion.

ABSTRACT:

Introduction

Naïve T cells remain in an actively maintained state of quiescence until activation by antigenic signals, upon which they start to proliferate and generate effector cells to initiate a functional immune response. Metabolic reprogramming is essential to meet the biosynthetic demands of the differentiation process, and failure to do so can promote the development of hypofunctional exhausted T cells.

Methods

Here we used 13C metabolomics and transcriptomics to study the metabolism of CD8+ T cells in their complete course of differentiation from naïve over stem-like memory to effector cells and in exhaustion-inducing conditions.

Results

The quiescence of naïve T cells was evident in a profound suppression of glucose oxidation and a decreased expression of ENO

SUBMITTER: Kirchmair A 

PROVIDER: S-EPMC10620935 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature