Production of a p65^fl/fl/LysMCre mouse model with dysfunctional NF-κB signaling in bone marrow-derived macrophages.

ABSTRACT: Here, we describe the production and characterization of a novel p65^fl/fl/LysMCre mouse model, which lacks canonical nuclear factor-kappaB member RelA/p65 (indicated as p65 hereafter) in bone marrow-derived macrophages. Cultured bone marrow-derived macrophages that lack p65 protein reveal NF-κB signaling deficiencies, a reduction in phagocytic ability, and reduced ability to produce nitrites. Despite abnormal bone marrow-derived macrophage function, p65^fl/fl/LysMCre mice do not exhibit differences in naïve systemic immune profiles or colony forming units and time to death following Salmonella infection as compared to controls. Additionally, p65^fl/fl/LysMCre mice, especially females, display splenomegaly, but no other obvious physical or behavioral di

SUBMITTER: Korkaya AK 

PROVIDER: S-EPMC10621469 | biostudies-literature | 2023 Nov

REPOSITORIES: biostudies-literature