Engineering a scalable and orthogonal platform for synthetic communication in mammalian cells.

ABSTRACT: The rational design and implementation of synthetic mammalian communication systems can unravel fundamental design principles of cell communication circuits and offer a framework for engineering of designer cell consortia with potential applications in cell therapeutics. Here, we develop the foundations of an orthogonal, and scalable mammalian synthetic communication platform that exploits the programmability of synthetic receptors and selective affinity and tunability of diffusing coiled-coil peptides. Leveraging the ability of coiled-coils to exclusively bind to a cognate receptor, we demonstrate orthogonal receptor activation and Boolean logic operations at the receptor level. We show intercellular communication based on synthetic receptors and secreted multidomain coiled-coils and demo

SUBMITTER: Makri Pistikou AM 

PROVIDER: S-EPMC10622552 | biostudies-literature | 2023 Nov

REPOSITORIES: biostudies-literature