Cardiolipin prolongs the lifetimes of respiratory proteins in Drosophila flight muscle.

ABSTRACT: Respiratory complexes and cardiolipins have exceptionally long lifetimes. The fact that they co-localize in mitochondrial cristae raises the question of whether their longevities have a common cause and whether the longevity of OXPHOS proteins is dependent on cardiolipin. To address these questions, we developed a method to measure side-by-side the half-lives of proteins and lipids in wild-type Drosophila and cardiolipin-deficient mutants. We fed adult flies with stable isotope-labeled precursors (¹³C₆¹⁵N₂-lysine or ¹³C₆-glucose) and determined the relative abundance of heavy isotopomers in protein and lipid species by mass spectrometry. To minimize the confounding effects of tissue regeneration, we restricted our analysis t

SUBMITTER: Ren M 

PROVIDER: S-EPMC10622840 | biostudies-literature | 2023 Oct

REPOSITORIES: biostudies-literature