Structure and function analysis of a type III preQ₁-I riboswitch from Escherichia coli reveals direct metabolite sensing by the Shine-Dalgarno sequence.

ABSTRACT: Riboswitches are small noncoding RNAs found primarily in the 5' leader regions of bacterial messenger RNAs where they regulate expression of downstream genes in response to binding one or more cellular metabolites. Such noncoding RNAs are often regulated at the translation level, which is thought to be mediated by the accessibility of the Shine-Dalgarno sequence (SDS) ribosome-binding site. Three classes (I-III) of prequeuosine₁ (preQ₁)-sensing riboswitches are known that control translation. Class I is divided into three subtypes (types I-III) that have diverse mechanisms of sensing preQ₁, which is involved in queuosine biosynthesis. To provide insight into translation control, we determined a 2.30 Å-resolution cocrystal structure of a class I type III pre

SUBMITTER: Schroeder GM 

PROVIDER: S-EPMC10622847 | biostudies-literature | 2023 Oct

REPOSITORIES: biostudies-literature