Preclinical study of CD19 detection methods post tafasitamab treatment.

ABSTRACT:

Introduction

Several CD19 targeted antibody-based therapeutics are currently available for patients with diffuse large B-cell lymphoma (DLBCL), including the Fc-modified antibody immunotherapy tafasitamab. This therapeutic landscape warrants the evaluation of potential sequencing approaches. Prior to a subsequent CD19-targeted therapy, CD19 expression on tafasitamab-treated patient biopsy samples may be assessed. However, no standardized methods for its detection are currently available. In this context, selecting a tafasitamab-competing CD19 detection antibody for immunohistochemistry (IHC) or flow cytometry (FC) may lead to misinterpreting epitope masking by tafasitamab as antigen loss or downregulation.

Methods

We analyzed a comprehensive panel of commercially available C

SUBMITTER: Ilieva K 

PROVIDER: S-EPMC10622958 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature