The molecular mechanism of propionate-regulating gluconeogenesis in bovine hepatocytes.

ABSTRACT:

Objective

Cows that are nursing get around 80% of their glucose from liver gluconeogenesis. Propionate, a significant precursor of liver gluconeogenesis, can regulate the key genes involved in hepatic gluconeogenesis expression, but its precise effects on the activity of enzymes have not yet been fully elucidated. Therefore, the aim of this study was to investigate the effects of propionate on the activity, gene expression, and protein abundance of the key enzymes involved in the gluconeogenesis of dairy cow hepatocytes.

Methods

The hepatocytes were cultured and treated with various concentrations of sodium propionate (0, 1.25, 2.50, 3.75, and 5.00 mM) for 12 h. Glucose content in the culture media was determined by an enzymatic coloring method. The activities of gluconeogen

SUBMITTER: Pang R 

PROVIDER: S-EPMC10623044 | biostudies-literature | 2023 Nov

REPOSITORIES: biostudies-literature