A comprehensive longitudinal study of gut microbiota dynamic changes in laying hens at four growth stages prior to egg production.

ABSTRACT:

Objective

The poultry industry is a primary source of animal protein worldwide. The gut microbiota of poultry birds, such as chickens and ducks, is critical in maintaining their health, growth, and productivity. This study aimed to identify longitudinal changes in the gut microbiota of laying hens from birth to the pre-laying stage.

Methods

From a total of 80 Hy-Line Brown laying hens, birds were selected based on weight at equal intervals to collect feces (n = 20 per growth) and ileal contents (n = 10 per growth) for each growth stage (days 10, 21, 58, and 101). The V4 regions of the 16S rRNA gene were amplified after extracting DNA from feces and ileal contents. Amplicon sequencing was performed using Illumina, followed by analysis.

Results

Microbial diversity incre

SUBMITTER: Choi S 

PROVIDER: S-EPMC10623045 | biostudies-literature | 2023 Nov

REPOSITORIES: biostudies-literature