Annotation of cell types (ACT): a convenient web server for cell type annotation.

ABSTRACT:

Background

The advancement of single-cell sequencing has progressed our ability to solve biological questions. Cell type annotation is of vital importance to this process, allowing for the analysis and interpretation of enormous single-cell datasets. At present, however, manual cell annotation which is the predominant approach remains limited by both speed and the requirement of expert knowledge.

Methods

To address these challenges, we constructed a hierarchically organized marker map through manually curating over 26,000 cell marker entries from about 7000 publications. We then developed WISE, a weighted and integrated gene set enrichment method, to integrate the prevalence of canonical markers and ordered differentially expressed genes of specific cell types in the marker

SUBMITTER: Quan F 

PROVIDER: S-EPMC10623726 | biostudies-literature | 2023 Nov

REPOSITORIES: biostudies-literature