BRD4/nuclear PD-L1/RelB circuit is involved in the stemness of breast cancer cells.

ABSTRACT:

Background

Breast cancer (BC) is the most common cancer diagnosed in women worldwide. BC stem cells (BCSCs) have been known to be involved in the carcinogenesis of the breast and contribute to therapeutic resistance. The programmed death-ligand 1 (PD-L1) expression of BC correlated with a poor prognosis. Immunotherapies that target PD-L1 have great potential and have been successful when applied to cancer treatment. However, whether PD-L1 regulates BCSC formation is unknown.

Methods

BCSCs were enriched by serum-free suspension culture. The properties of BCSCs were examined by mammosphere formation assay, CD44⁺/Cd24^-, aldehyde dehydrogenase (ALDH) assay, CSC marker analysis, and mammosphere growth assay. To elucidate the functions of bromodomain-containi

SUBMITTER: Kim SL 

PROVIDER: S-EPMC10623882 | biostudies-literature | 2023 Nov

REPOSITORIES: biostudies-literature