Proteomic profiling of urinary extracellular vesicles differentiates breast cancer patients from healthy women.

ABSTRACT: Urinary extracellular vesicles (uEVs) reflect the biological conditions of the producing cells. The protein profiling of uEVs allow us to better understand cancer progression in several cancers such as bladder cancer, prostate cancer and kidney cancer but has not been reported in breast cancer. We have, herein, aimed at quantifying the concentration and at generating the proteomic profile of uEVs in patients with breast cancer (BC) as compared to that of healthy controls (CT). Urine samples were collected from 29 CT and 47 patients with BC. uEVs were isolated by using differential ultracentrifugation, and were then characterized by Western blotting and transmission electron microscopy. Moreover, a nanoparticle tracking analysis was used in order to measure the concentration and the size di

SUBMITTER: Jeanmard N 

PROVIDER: S-EPMC10624262 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature