Circulating antibody-secreting cells are a biomarker for early diagnosis in patients with Lyme disease.

ABSTRACT:

Background

Diagnostic immunoassays for Lyme disease have several limitations including: 1) not all patients seroconvert; 2) seroconversion occurs later than symptom onset; and 3) serum antibody levels remain elevated long after resolution of the infection.

Introduction

MENSA (Medium Enriched for Newly Synthesized Antibodies) is a novel diagnostic fluid that contains antibodies produced in vitro by circulating antibody-secreting cells (ASC). It enables measurement of the active humoral immune response.

Methods

In this observational, case-control study, we developed the MicroB-plex Anti-C6/Anti-pepC10 Immunoassay to measure antibodies specific for the Borrelia burgdorferi peptide antigens C6 and pepC10 and validated it using a CDC serum sample collection. Then we examin

SUBMITTER: Haddad NS 

PROVIDER: S-EPMC10624293 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature