Hsa_circ_0001946 Ameliorates Mechanical Stress-induced Intervertebral Disk Degeneration Via Targeting miR-432-5p and SOX9.

ABSTRACT:

Study design

Experimental analysis of circular RNA in intervertebral disk degeneration (IDD).

Objective

This study aimed to explore the roles of hsa_circ_0001946 (circ-CDR1as) in mechanical stress-induced nucleus pulposus cell injury in IDD.

Summary of background data

Mechanical stress is an important pathogenic factor for IDD. Excessive compression stress leads to nucleus pulposus (NP) cell apoptosis and extracellular matrix (ECM) degradation and accelerated IDD. Circ-CDR1as is associated with various degenerative conditions, but its role in IDD is not clear. Herein, we explored the roles and mechanisms of circ-CDR1as in IDD in vitro.

Materials and methods

An in vitro model of IDD was constructed by treating NP cells with 1.0 MPa compression stress. Quantitati

SUBMITTER: Xiang Q 

PROVIDER: S-EPMC10624407 | biostudies-literature | 2023 Aug

REPOSITORIES: biostudies-literature