Scalable Generation of Pre-Vascularized and Functional Human Beige Adipose Organoids.

ABSTRACT: Obesity and type 2 diabetes are becoming a global sociobiomedical burden. Beige adipocytes are emerging as key inducible actors and putative relevant therapeutic targets for improving metabolic health. However, in vitro models of human beige adipose tissue are currently lacking and hinder research into this cell type and biotherapy development. Unlike traditional bottom-up engineering approaches that aim to generate building blocks, here a scalable system is proposed to generate pre-vascularized and functional human beige adipose tissue organoids using the human stromal vascular fraction of white adipose tissue as a source of adipose and endothelial progenitors. This engineered method uses a defined biomechanical and chemical environment using tumor growth factor β (TGFβ) pathway inhibitio

SUBMITTER: Escudero M 

PROVIDER: S-EPMC10625054 | biostudies-literature | 2023 Nov

REPOSITORIES: biostudies-literature