Circulating miRNAs signature on breast cancer: the MCC-Spain project.

ABSTRACT:

Purpose

To build models combining circulating microRNAs (miRNAs) able to identify women with breast cancer as well as different types of breast cancer, when comparing with controls without breast cancer.

Method

miRNAs analysis was performed in two phases: screening phase, with a total n = 40 (10 controls and 30 BC cases) analyzed by Next Generation Sequencing, and validation phase, which included 131 controls and 269 cases. For this second phase, the miRNAs were selected combining the screening phase results and a revision of the literature. They were quantified using RT-PCR. Models were built using logistic regression with LASSO penalization.

Results

The model for all cases included seven miRNAs (miR-423-3p, miR-139-5p, miR-324-5p, miR-1299, miR-101-3p, miR-186-5p an

SUBMITTER: Gomez-Acebo I 

PROVIDER: S-EPMC10625260 | biostudies-literature | 2023 Nov

REPOSITORIES: biostudies-literature