SFgl2 gene-modified MSCs regulate the differentiation of CD4⁺ T cells in the treatment of autoimmune hepatitis.

ABSTRACT:

Background

Autoimmune hepatitis (AIH) is a T-cell-mediated autoimmune liver disease that can lead to liver injury and has a poor long-term prognosis. Mesenchymal stromal cells (MSCs) have immunosuppressive effects and can treat AIH. CD4⁺ T cells express the unique inhibitory Fcγ receptor (FcγRIIB), which is the only receptor for the immunosuppressive factor soluble fibrinogen-like protein 2 (sFgl2). This study aimed to examine the therapeutic effect of sFgl2 gene-modified MSCs (sFgl2-MSCs) on AIH.

Methods

MSCs were obtained from the inguinal fat of mice and cocultured with CD4⁺ T cells sorted from mouse spleens. FcγRIIB expression on CD4⁺ T cells was determined by flow cytometry. sFgl2 expression in MSCs transfected with lentiviral vectors ca

SUBMITTER: Ji W 

PROVIDER: S-EPMC10625288 | biostudies-literature | 2023 Nov

REPOSITORIES: biostudies-literature